Con A dishes

Concanavalin-coated coverslip dishes

• Start with 5 mg/mL ConA stock solution

• Keep sterile - work in sterile hood

• aliquot 100 µL into microfuge tubes  (cannot take repeated freeze-thaw cycles)

• Coat coverslip bottom dishes with either:

  • 0.75 mL 0.1 mg/mL:  0.015 mL (5 mg/mL) + 0.735 mL PBS
  • 0.75 mL 0.5 mg/mL:  0.075 mL (5 mg/mL) + 0.675 mL PBS

• Incubate the coverslip dishes for 1 hour 37˚C 

• Remove the solution

• rinse in PBS 

• Air dry the dish.