Thyroid hormones

Submitted by kdorfman on Fri, 09/06/2019 - 18:38

for Fish

5 mL per well for treatment

PTU (inhibitor) Sigma p3755
MW 170.2
Solubility:

  • 50 mg/mL in 1M NaOH
  • 16 mg/mL in alcohol
  • 1 mg/mL in RT water
  • 10 mg/mL in boiling water

In order to get the following doses, best to make it in hot fish water; otherwise, the NaOH or EtOH will be too concentrated in the final dilution.

  • dose 1: 0.5 mM

  • dose 2: 1.0mM

MW of PTU:
Concentration: M
to make: mL
add: g PTU

L-T4 (thyroxin) Sigma T2376
MW: 776.87
solubility:

  • 50 mg/ml in 4M NH4OH in MeOH
  • 0.5 mg/mL in 1:5 DMSO:PBS

Solution is good for ~ 1 day.

Make a 1 mM stock in 1M NaOH. Takes a long time. Vortex.

Priya's recipe: 1 mg/mL in 1M NaOH (~25.7 uM stock)

Working concentrations

  • dose 1: 100 nM (=0.1 uM)

  • dose 2: 300 nM (=0.3 uM)

MW of T4:
Concentration: M
to make: mL
add: g T4