3.3: Direct fluor

Submitted by kdorfman on Tue, 12/06/2011 - 15:57

Direct Fluorescent Labeling of Organelles


CHECK THIS FOR HBS vs PBS!!


Cells

3t3, LLCPk

  • Fixed in paraglut for phalloidin
    • 8 3t3 (can be fixed as late as the morning of lab)
    • 8 LLCPk (can be from pre-semester prep)
  • Live on small diameter MatTek dishes for Mitotracker and ceramide
    • 8 3t3
    • 8 LLCPk

Reagents