1.2 DNA Quantification

Submitted by margaret on Wed, 11/16/2011 - 18:07

Goal for this lab:

Quantify Arabidopsis DNA by two methods. (It is hard to finish all the analysis in 3 hours – better if this happens on a Monday 4 hour lab. Otherwise save the gel analysis till the next period, along with the Wiki.)

For pairs of students
make: 1 mL aliquots DB (10 mM Tris pH 7.5) or T10E1
make: 100 µL aliquots 6X loading dye
make: 25 µL aliquots MassRuler High Range

6X Loading dye to be used throughout the semester: 100 µL

MassRuler™ DNA Ladder, High Range Fermentas #SM0393 Give them 25µL in a microfuge tube