G&GA solutions

Make solutions (Check supplies first)

See stock solution recipes


Aliquot:

Solution vol aliquots/pair total aliquots for Labs
T10E1 1 mL 5 50 1.1, 1.2, 2.4, 3.3
T10E5 0.75 mL 2 20 1.1
EtOH 95% 1.5 mL 2.2 22 1.1, 5.4
EtOH 70% 7.5 mL 1.2 12 1.1
isopropyl 7.5 mL 1.2 12 1.1
NaOAc 100 µL 1.2 12 1.1
KOAc 5 mL 1.2 12 1.1
DEB 10 mL 1.2 12 1.1
Tris pH 7.5 10 mM 1.5 mL 3 30 1.2, 5.4
loading dye 50 µL 1.5 15 1.2, 2.4, 2.5, 3.4, 5.4, 5.6
HMW std 15 µL 1.2 12 1.2
dNTP 2.5 mM 150 µL 1.5 15
sterile H2O 1 mL 6 60 2.4, 2.5, 3.3, 5.5
LMW std 50 µL 1.5 15 2.4, 2.5, 3.4, 5.4, 5.6
AttachmentSize
fermentas_calcs.xls22 KB