Staining solution NucBlue + Mitotracker

Submitted by kdorfman on Fri, 09/30/2022 - 13:47

Staining Solution

  • 1 mL fluorobrite
  • 10uL NucBlue
  • 1 uL Mitotracker red

Staining Protocol

  • Remove pink medium from cells (~2.5 mL)
  • Replace with Staining Solution
  • Rock dish to make sure solution covers the surface
  • Incubate ~15 min at 37C

Observation Protocol

  • Find cells with phase
  • Switch to fluorescence
    • turn on arc lamp
    • turn off white light
    • open mechanical shutter (CLOSE WHEN NOT LOOKING AT CELLS! THEY BLEACH!)
    • open electronic shutter
    • turn filter cube to UV to see blue nuclei
    • turn filter cube to G to see red mitochondria