Staining solution NucBlue + Mitotracker

Staining Solution

• 1 mL fluorobrite
• 10uL NucBlue
• 1 uL Mitotracker red

Staining Protocol

• Remove pink medium from cells (~2.5 mL)
• Replace with Staining Solution
• Rock dish to make sure solution covers the surface
• Incubate ~15 min at 37C

Observation Protocol

• Find cells with phase
• Switch to fluorescence
  • turn on arc lamp
  • turn off white light
  • open mechanical shutter (CLOSE WHEN NOT LOOKING AT CELLS! THEY BLEACH!)
  • open electronic shutter
  • turn filter cube to UV to see blue nuclei
  • turn filter cube to G to see red mitochondria