Fluo-4

Fluo-4 stock
Invitrogen F14217 500 µL
1mM in DMSO
Protect from light
Store in dissicator.
20 µL aliquots. Each makes 10 mL of 2 µM solution

Fluo-4 staining solution
2 µM Fluo-4 + 0.02% pluronic in HBS

Make enough for 7.1 and 7.2: 250 µL x 4 expts x 8 grps x 2 days = 16 mL.
Make 20 mL
Need 16 aliquots, each ~1.05 mL

ingredient 5 10 15 20 mL
1 mM Fluo-4 stock 10 20 30 40 µL
20 % w/v Pluoronic 5 10 15 20 µL

plus HBS to final volume