3.2: Immunofluor

Immunofluorescence Labeling

Turn on the Incubator

Reagents

  • PBS (warm) for rinsing medium off cells before fixation

  • PBS-Tw-Az for rinsing coverslips (in carboy)

  • 2% BSA in PBS to make antibodies

  • Fixative

    • paraglut for students
    • methanol in freezer for certain antibodies
  • Slides

  • Nail polish

Mystery Antibodies for 2012

No. Antibody from fixative secondary filter cube
1 Golgi KD formaldehyde goat anti mouse G
2 LAP2 PW methanol goat anti mouse G
3 gamma tub PW paraglut or form goat anti rabbit G
4 ZO1 KD methanol or paraform goat anti rabbit G
5 vinculin PW formaldehyde goat anti mouse G