5.1: Motility

Motility

Live cells on MatTek dishes

8 MatTek dishes each:

  • 3t3

  • B16 (do more than one set, at different concentrations)

  • LLCPK actin

Must be plated lightly for motility.

Scheduling

  • Thaw LLCPK-actin, B16 24 hours before plating

  • Plate on all cells (lightly) Monday for Wednesday lab

3t3’s must have 36-48 hours to stretch out.

If they get overgrown (especially B16s), scrape some off

Reagents

Aliquot non-CO2, serum-free medium