Lab 4.2 prep

10/28 & 11/2 2021

Make 12 tubes of 12 media, 5 mL each.

6 sets per day.

Day before:

• make LB-glu (mix 20% glucose from genetics 1:1 with LB if necessary)
• Start overnight culture in LB-glu

Day of:

• Borrow Biochem multi-channel pipet plus small tips. Dial down to 5 uL
• Subculture into LB glu again
• Just before use, wash with LB 3x
• Resuspend in 10 mL LB
• Need OD = 0.02 in the wells
  • Need 10 mL to put in sterile petri dish to fill pipet
  • Need an OD that doesn't appreciably raise the OD of 205 uL LB (OD =~0.2)
  • Aim for blank corrected OD of 0.8
  • Dilute as necessary