SC minus (adenine, histidine, leucine, tryptophan)
Jeff's recipe: 10X SC –AHLT 16.4 g/L
Dissolve 16.4 g of Sunrise Science SC –Adenine, –Histidine, –Leucine and –Tryptophan in 1 L distilled water and filter sterilize.
Store in the dark at 4 ˚C.
Will not go into solution.
From the manufacturer:
Commonly added to YNB, nitrogen and glucose, at suggested g/L, for a complete yeast media. Mix with stirring for 10-15 minutes, and filter sterilize or autoclave at 121°C for 15 minutes. To prepare plates, autoclave agar separately and add to sterile medium, or add agar to liquid medium and adjust to pH 5.8-6.0 before autoclaving.
Suggested g/L: 1.64
Storage Temperature: 2-8 C
4X will dissolve. Almost completely clear after overnight stirring. Crystal clear after autoclaving.