MacConkey Agar
- Fisher 212122 2 kg (Difco)
- Krackeler 10-211387 500g (via Sigma)
- HiMedia MacConkey agar, granulated GM081 (Fisher NC1876763, VWR 95020-204)
to distinguish Lac+ and Lac- bacterial strains.
50 g/L
(If you are going to make more than one bottle, turn on 65C waterbath in tissue culture room)
Turn on the stir-plate heat to 105C.
Withhold 50 mL of water to rinse the mouth of the bottle.
Heat the rest of the water to boiling in the bottle in the microwave.
Measure out the agar mix while the water is heating
Add the stir bar (CAREFULLY!) to the bottle of hot water
Put the bottle on the stir-plate
Add the agar mix to the bottle
Use the reserved water to rinse the granules stuck in the neck down to the liquid.
Stir and heat until granules are completely dissolved.
Cap loosely, wrap with foil, and autoclave.
(If you make more than one bottle, the extras go in the water bath till you are ready to pour)
leave stir bar in
put bottles on stir plate near sterile hood until handle-able
If you accidentally buy
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