MacConkey + bile+ CV

Submitted by kdorfman on Mon, 01/08/2024 - 16:00

If you accidentally buy MacConkey without

  • bile salts (1.5 g/L)
  • Crystal violet (0.001g/L)
  • NaCl (5 g/L)
To make Maconkey agar plates
at mm plate diameter
pour mL per plate
start with: mL hot water
add g Maconkey agar (without bile, CV, NaCl)
add g bile salts
add g NaCl
add mL 1% crystal violet solution, heat to boiling, stir till dissolved
autoclave for: minutes