P-Glo Bacterial media

Submitted by kdorfman on Mon, 08/26/2019 - 22:26

Make 60 mm plates

2019 - for QBoC:

  • transformed pglo plasmid (1660405EDU) from the BioRad kit into
  • DH5 alpha E. coli (Invitrogen 18265-017) from the -80
  • Lyophilized cells (BioRad 1660408EDU) did not arrive in time

Used the Invitrogen protocol.

Plated on:

  • LB
  • LB amp
  • LB amp ara

Saw gfp colonies on the arabinose plates. Collected, grew up in LB amp, froze in 15% glycerol.