DNA from cultured cells

Submitted by kdorfman on Tue, 08/22/2023 - 16:04

From QIAamp DNA Mini & Blood Mini Handbook, Apendix B, p 50

Do not use more than 5 x 10^6 cells

Important points before starting

  • Do not use more than 5 x 106 cells (with a normal set of chromosomes).
  • All centrifugation steps are carried out at room temperature (15–25°C).
  • Use carrier DNA if the sample contains <10,000 genome equivalents (see page 17).

Things to do before starting

  • Heat a water bath or heating block to 56°C.
  • Equilibrate Buffer AE or distilled water to room temperature (15–25°C) for elution.
  • Ensure that Buffer AW1, Buffer AW2, and QIAGEN Protease have been prepared according to the instructions on page 16.
  • If a precipitate has formed in Buffer AL, dissolve by incubating at 56°C.