Dog DNA labs, week of 10/23/23
Isolate DNA
1 sample per pair, from:
- Dog cell cultures from Kathleen
- Student dog mouth swabs
- DNA from Cornell BioBank
- Staff dog mouth swabs
QIAamp DNA minikit 51306
Equipment
- Microfuge tubes
- Sterile forceps (to pull swab from extraction buffer)
- QIAamp columns
- collection tubes
- 56C heat block (10 min)
- vortex mixers
- centrifuge 6000 x g 1 min, 20K x g 3 min
- Nanodrop (may need next class, also) (Need to calculate uL to get 100 ng)
Reagents (per swab)
- PBS (400 UL)
- Proteinase K (20 uL)
- Buffer AL (400 uL)
- 95% EtOH (400 uL)
- QIAamp Mini spin column (1)
- 2 mL collection tube (2?)
- Buffer AW1 (500 uL)
- Buffer AW2 (500 uL)
- Buffer AE (50 uL)
Staff makes spreadsheet on Google Drive with:
- sample # for each dog
- concentration
- quality
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