Week 08 DNA extraction

Submitted by kdorfman on Mon, 07/24/2023 - 20:25

Dog DNA labs, week of 10/23/23

Isolate DNA

1 sample per pair, from:

QIAamp DNA minikit 51306

Equipment

  • Microfuge tubes
  • Sterile forceps (to pull swab from extraction buffer)
  • QIAamp columns
  • collection tubes
  • 56C heat block (10 min)
  • vortex mixers
  • centrifuge 6000 x g 1 min, 20K x g 3 min
  • Nanodrop (may need next class, also) (Need to calculate uL to get 100 ng)

Reagents (per swab)

  • PBS (400 UL)
  • Proteinase K (20 uL)
  • Buffer AL (400 uL)
  • 95% EtOH (400 uL)
  • QIAamp Mini spin column (1)
  • 2 mL collection tube (2?)
  • Buffer AW1 (500 uL)
  • Buffer AW2 (500 uL)
  • Buffer AE (50 uL)

Staff makes spreadsheet on Google Drive with:

  • sample # for each dog
  • concentration
  • quality