Master Mix, multiple primers

Submitted by kdorfman on Mon, 07/01/2024 - 22:10

NOT SURE WHY THIS DOESN"T CALCULATE

To make Qiagen Master Mix + primers for students (or pairs of students)
and reactions per student (or pair)
and primer sets (R + F)
at uL per reaction
Start with: uL Qiagen Master Mix
add: uL of each F and R 100 uM primer
add: uL water
for a final volume of: uL Qiagen Master Mix (including primers!)
aliquot: uL Qiagen Master Mix (including primers!) per student (or pair)