| To make Qiagen Master Mix + primers for | students |
| and | reactions per student |
| at | uL per reaction |
| Start with: | uL Qiagen Master Mix |
| add: | uL forward eGFP primer (100 mM) |
| add: | uL reverse eGFP primer (100mM) |
| add: | uL forward Hedgehog Inhibitory Protein primer (100 mM) |
| add: | uL reverse Hedgehog Inhibitory Protein primer (100 mM) |
| add: | uL water |
| for a final volume of: | uL Qiagen Master Mix plus primers |
| aliquot: | uL Qiagen Master Mix plus primers per student |
