Plate Reader Programs

Plate Reader Programs kdorfman Thu, 10/13/2022 - 15:20

Look for Programs here

Desktop/This PC/Local Disc (C:)/Users/Public/Public Downloads/PS1_Protocols

or

On Pstar2, even though the files say Polar star 1):

Desktop/This PC/Local Disc (C:)/Users/Public/Public Downloads/polarstar1/PS1_Protocols

Program Layout method ex em vol
BUG OD 1 A&B Abs 595 . 250
BUG_FLUOR 2 A:H FL 486 520 .
BUG_OD_SHAKE 3 A:H ABS 595 . 200
FL-ABS-BY-2 4 C1:2, D ABS 485 . 150
REPEATABILITY 5 A & B ABS 485 . 150
SERIAL2&5_ABS 6 E1-5, F:H ABS 485 . 150
SERIAL2&5_FL 7 E1-5, F:H FL 485 520 .
REPEATAILITY_FL 8 A:B FL 485 520 .

Script for overnight growth and fluorescence

helpful video from BMG

  1. Lab 3.1 (2022) counting bacteria: std curve OD vs population density ↩︎

  2. Lab 4.2 (2022) lac operon shake cells, then read OD for overnight growth curves – part of script “Grow&Glow” ↩︎

  3. Lab 4.2 (2022) lac operon after BugOD Shake,read FL for overnight growth curves– part of script “Grow&Glow” ↩︎

  4. Lab 1.2 serial dilution & plate reader sensitivity ↩︎

  5. Lab 1.2 pipetting consistency (ABS) ↩︎

  6. Lab 1.3 serial dilution; absorbance of fluorescein; std curve ↩︎

  7. Lab 1.3 serial dilution; fluorescence of fluorescein; std curve ↩︎

  8. (Lab 1.2) pipetting consistency (FL) not used in 2022 ↩︎

Script for lac operon lab

Script for lac operon lab kdorfman Mon, 10/24/2022 - 15:18

Script for overnight growth and fluorescence

Set Preferences inside the user dialog box before running a script!

Location: C:\Users\Public\Downloads\PS1_Protocols\E_Coli_Grow&Glow.btc

(or PS2)

Multiple measurements for 17.5 hours

;absorbance and fluorescence

st1:="BUG_OD_SHAKE"

st2:="BUG_FLUOR"

for i:=1 to 70 do begin

ID1:="BOD1"

ID2:= <protocol>

ID3:=<method>

R_Run "<st1>"

ID1:="BFL1"

ID2:= <protocol>

ID3:= <method>

R_Run "<st2>"

wait for 13 m

end;

beep

(Names for PS2 = BOD2, BFL2)