Media for Lac I

LB (150 mL)

LB +

  • 0.1mM IPTG (60 mL)

  • 0.5 M lac (60 mL)

  • 0.5 M glu (80 mL)

  • 0.5 M gal (60 mL)

  • 0.5 M mal (40 mL)

Make enough for Lac operon I and II:

150 mL each + 50µg/mL Kan

  • lac = 27 g

  • gal = 13.5 g

  • mal = 27 g

  • IPTG = 0.3 mL 100mM stock

200 mL glu = 18 g

Kan calculation here:

https://wahoo.nsm.umass.edu/content/media-lab-31