2015

Make enough media for 3.1 (25 mL) and 3.2 (200 mL of LB, 150 Lac, 150 glu, 100 each of the others)

Calculate amount of each medium assuming they will make mixtures in 2 mL tubes. Each group needs ~12 mL. Mostly LB, then lac, then glu

for 3.1:

  • Each pair gets 1 set of 12 tubes, each with E. coli already grown up. LB +

    • nothing
    • lac (0.25 M)
    • IPTG (0.01mM)
    • glu (0.25 M)
    • gal (0.25 M)
    • mal (0.25 M)
    • IPTG + glu
    • IPTG + mal
    • lac + glu
    • lac + mal
    • gal + glu
    • gal + mal
  • Overgrown cultures all are too fluorescent!

  • Can use the same tubes for both lab sections (if students don't throw them out)
  • Night before: grow up GFP E coli from liquid culture in ~25 mL
  • morning of: aliquot cultures into tubes 1mL each.