Cytochalasin D

Submitted by kdorfman on Mon, 04/23/2018 - 18:21

MW = 507.62
Sigma C2618: 200 µL 5 mg/mL (~10 mM) in DMSO
Sigma C8273: 5 mg. Dissolve in 1 mL DMSO

  • alkaloid mycotoxin produced by Helminthosporium and other molds.
  • disrupts actin microfilaments
  • binds to F-actin polymer
  • prevents polymerization of actin monomers
  • Aliquot 10 µL 10 mM
  • Add 90 uL DMSO (makes 1 mM)
  • Dilute this 1:1000 into medium (makes 1uM)

  • Dilute the 1 uM to desired working concentration

  • Store in the dark, in the freezer

  • Adding 490 µL of non-CO2 medium makes 500 µL of 200 µM

  • Final dilution should contain no more than 0.1% DMSO

  • 250 nM in the dish should give an effect

  • 15 - 30 min

c2618dat.pdf