Link to Programs here
sign in to the "student" account
user = .\student
pw = student@ISB364
Find Optima Control in the start menu
Folders icon pinned to taskbar
Data files go to: This PC > Local Disc (C:) > users > student > documents
(also to backups)
Find protocols to import in: This PC > Local Disc (C:) > users > Public > Public Downloads > PS1_Protocols
Plate IDs (in the run menu)
ID1 is file name. Be sure to give a file name
To get the meta data on the output csv file:
Setup
Program configuration
preferences
define format
Set Preferences before running a script! See p 28 of user manual ii, below.
Setup > Program Configuration > Define Format > Filename and Path
File info
Overwrite, append, make new file with same name + number
Header:
- no header - just the data - useful for files that have to be compiled
- long header includes wavelength - just the right amount of info (USE THIS)
- full header (Ex, Em wavelength, date, time, etc) way too much info
- short header does not include wavelength
- Danish headers writes the file name next to the row ID. (!?!)
Style
- Table: no well numbers, or wavelength indicators, etc. Just the results in plate layout form.
- Table with well numbers: puts the well IDs next to the reading (Very hard to read)
- Table with well numbers (only measured wells)
Table with well numbers in plate layout style: USE THIS
List (Literally, a list. no plate info. all values in one column)
List with well numbers
A B 1 A01 10 2 A02 12 3 A03 11 4 A04 13 List with well numbers (only measured wells): puts a - for a skipped well)
- List sorted by wells (all cycles in one row, chromatics in separate blocks)
- List sorted by wells with well numbers
- List sorted by well numbers (only measured wells)
- List sorted by wells (all cycles/intervals/channels/chromatics in one row)
- List sorted by wells 2 " " " (only measured wells)
Script mode
For i = 1 to 72 This is the counter
id 1 = "BOD1" BOD1 is the file name
id 1 = "BOD1" i makes a separate file for each run, called BOD1_1. BOD1_2 etc.
Excitation filters | Emission filters |
---|---|
340 | 520 |
485 | 570 |
492 | 590 |
530 | 620 |
544 | |
584 | |
595 |
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