LB (150 mL)
LB +
0.1mM IPTG (60 mL)
0.5 M lac (60 mL)
0.5 M glu (80 mL)
0.5 M gal (60 mL)
0.5 M mal (40 mL)
Make enough for Lac operon I and II:
150 mL each + 50µg/mL Kan
lac = 27 g
gal = 13.5 g
mal = 27 g
IPTG = 0.3 mL 100mM stock
200 mL glu = 18 g
Kan calculation here:
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