Taq dilution buffer

Submitted by kdorfman on Fri, 05/31/2013 - 17:21

To dilute Takara Ex-Taq

Store in freezer. Won’t freeze solid. Should keep forever.

To make mL Taq dilution buffer
add: mL Tris 1M pH 8
add: mL KCl 1M
add: mL EDTA 0.5M
add: mL DTT 1M
add: mL Tween 20
add mL Nonidet P-40
add mL glycerol

bring to final volume, filter sterilize in BSC, freeze