Kanamycin

50 mg/mL stock (50 µg/mL in medium)

Concentration in poured agar drops to ~80% in 1 week, but stays stable for 3 more weeks at 4C

1.25 g in 25 mL H2O

filter sterilize

mL aliquots

freeze

1.2 mg/mL for QSB

0.84 mL 50 mg/mL stock

34.16 mL water

filter sterilize (if any doubt about sterility)

48 0.7 mL aliquots

freeze