Fast Plants Labs 2023-F
Fast Plants Labs 2023-F kdorfman Thu, 07/20/2023 - 18:08Here are the prep pages for the Fast Plants genetics labs 2023
Fast Plant Resources
Fast Plant Resources kdorfman Thu, 07/20/2023 - 22:00Here are some resources for growing and working with Fast Plants
Purple stem/yellow green leaf stock document
Fast Plants Growing Instructions from Carolina
Fast Plants Life cycle
Fast Plants Life cycle kdorfman Thu, 07/20/2023 - 19:14Interactive life cycle illustration
Day | developmental event(s) |
---|---|
1&2 | germination; seed swells with water until its coat cracks |
3 | hypocotyl pushes through soil, pulling cotyledons with it; seed coat falls off |
4 | hypototyl elongates above soil; roots grow down and anchor plant |
5-8 | True leaves develop; root hairs grow |
9-13 | Flower buds |
14-17 | Flowers open; pollination is possible |
18-20 | Fertilized eggs inside pistils grow to become embryos; pistil swells to become pod |
12-40 | Petals wilt and fall off; pods dry out. (Stop watering to encourage pod maturation.) When they are completely dry, seeds can be harvested |
Fast Plant overall crossing scheme
Fast Plant overall crossing scheme kdorfman Wed, 08/02/2023 - 16:00allele | trait |
---|---|
ANL | purple stem |
anl | green stem (anthocyanin-less) |
YGR | green leaves |
ygr | yellow-green leaves |
Generation | genotype | X | genotype | notes |
---|---|---|---|---|
P | anl/anl (anthocyaninless) | X | ygr/ygr (yellow-green leaf) | we plant demos |
F1 | ANL/anl YGR/ygr | X | ANL/anl YGR/ygr | purchased from Carolina |
F2 | 9 ANL/__ YGR/__: 3 ANL/___ /ygrygy : 3 anl/anl YGR/___ : 1 anl/anl ygr/ygr |
grown up from our F1 x F1 pollination |
Week 03 Planting Fast Plants
Week 03 Planting Fast Plants kdorfman Thu, 07/20/2023 - 18:14Fast Plants Day 1 (week of 9/18/23):
(See also Yeast genetics 2)
Students plant seeds of F1 Non-Purple Stem, Yellow-Green Leaf (anl/ANL, ygr/YGR)
These are the offspring of two P1 strains:
Non-Purple Stem, Hairless (anl/anl) X
Yellow-Green Leaf (ygr,ygr)
We plant at least 6 pots of non-purple stem parents (P1: anl/anl) for a demo, and ask students to puzzle out the genetics of the other parent "Who's the parent?" One pot per table.
- 1 pot per student
- place an inner "mesh" tray into the sink
- place bucket of soil and an empty tray with mesh insert on cart next to sink
place a filled bucket of water by the sink
per pair:
- 15 mL tube of fertilizer pellets (fill tube to ~2ml)
- microfuge tube of F1 seeds (up to 10 seeds/pair)
- little paint brush
- weigh boat
- labeling tape and marker
According to Carolina,
"F1 generation seed will express dominant traits (purple stems, dark green leaves, standard height) and can be used to produce F2 seed that will show the typical monohybrid (3:1) or dihybrid (9:3:3:1) phenotypic ratios. F2 seed will express the appropriate phenotypic ratios."
Rose pots 4x9 in a flat, Instructions to students:
- Use tape to label pots (Section #, Student Pair #, Date, Name)
- Fill 1/3 with potting soil (contain activity inside soil bucket)
- add 8 fertilizer pellets, do not mix
- fill to the top with soil (work inside soil bucket, do not mix)
- push down soil
- put pot in mesh tray in sink, hold down
- add water from a cup to settle the soil
- put up to 5 seeds on soil surface (hoping for min 1 plant/pot)
- cover with dusting of soil
- put in new tray that contains a mesh insert
Instruction to TAs:
- keep (or place) mesh tray of all collected pots in the sink
- water gently, carefully ensuring seeds remain at the top surface of the soil
- ensure soil is packed and moist enough for seeds to germinate
- when different seed genotypes are used, take care not to transfer any seeds between pots
- after excess drains into sink, move into a tray and cover with clear dome lid
- carry tray to growth chamber
Grow in growth chamber in ISB373: 24 hour light, 24C
Water from the bottom (into the tray, not into the pots), as needed
Week 04 F1 Seedling observations
Week 04 F1 Seedling observations kdorfman Mon, 07/24/2023 - 16:34Fast Plants Labs week of 9/23/23
(See also micropipetting and yeast DNA extraction)
See the stock document about observations
Transfer inner slotted tray to a dry flat before transport to classroom
All plants should all be purple stem, green leaves
Make a prediction about P2
Moodle doc "Fast Plants Observations After One Week" has questions for students while they look at plants.
Week 05 Pollination
Week 05 Pollination kdorfman Mon, 07/24/2023 - 16:46Fast Plant Labs week of 10/2/23
See also
F1 x F1 pollination
Remove the rare non-purples before class!
(Not the parents we planted!)
Everyone pollinates every plant. (Staff repeats afterward for complete mixing of pollen)
Non-sterile cotton swab.
Week 06 - no labs
Week 06 - no labs kdorfman Wed, 08/09/2023 - 19:50No Labs - indigenous peoples day
Lab makeups as needed
Week 07 & 08 Plant observations
Week 07 & 08 Plant observations kdorfman Mon, 07/24/2023 - 16:46Fast Plant Labs weeks of 10/16 and 10/23 2023
(See also Yeast DNA sequencing, )
Observe F2 seedlings
Enter F2 data by pair
Week 09 Collect & Plant F2 Seeds
Week 09 Collect & Plant F2 Seeds kdorfman Mon, 07/24/2023 - 16:47Week 10 F2 observations
Week 10 F2 observations kdorfman Mon, 07/24/2023 - 16:55Week 11 - work on reports
Week 11 - work on reports kdorfman Mon, 07/24/2023 - 16:56Fast Plant labs week of 11/13/23
(See also dog TRYP1 locus, analysis of MC1R genotype & sequence, cap3 sequence assembly)
Compile & analyze data
Week 12 - no labs
Week 12 - no labs kdorfman Wed, 08/09/2023 - 19:5111/20 - week of Thanksgiving
Week 13 Plant reports due
Week 13 Plant reports due kdorfman Mon, 07/24/2023 - 16:57Turn in Fast Plants report week of 11/27/23
Turn in report on Fast Plant genetics experiment
See also Dog genes bioinformatics