Week 11 Restriction digest

Submitted by kdorfman on Mon, 07/24/2023 - 20:27

Dog DNA labs, week of 11/13

Genotyping loci B (TyRP1), E (MC1R), K(defb103)

We provide folder with sequences

Restriction digest TyRP1 ("B" locus) 1 uL/rxn each:

We provide uncut


  • 4peaks to clean sequences Ma, Mb, Mc
  • CAP3 to assemble contigs to get MC1R sequence
  • Clustal also!
  • BLAST to compare to known nt sequences: MC1R (E), Defb103 (K)
  • Expasy (nucleotide to amino acid sequence)
  • Clustal omega to align aa to known sequences