Primers

Fisher Custom Oligos

  • Primers come lyophilized.

  • Tube label says how many nmol in the tube (usually ~100-500)

  • Multiply nmol x 10 = μL of sterile water to add to make 100 μM stock.

  • This is the only stock!

  • Put it away safely on instructor shelf in freezer.

  • Make a working stock for students (give them all of it):

  • 12.5 μM (12.5 μL of the 100 μM stock + 87.5 μL water).

(If using the repeater pipet for the water, 85 µL water + 12.14 µL 100 µM stock)