Media for 5.2

Minimal medium + HMLTT.

Enough to grow overnight cultures and make dilute early log phase cultures of all 7 chemotaxis strains for use during lab.

Swarm Agar

ingredient 750 1000 1500 mL
tryptone 7.5 10 15 g
NaCl 3.75 5 7.5 g
bacto-agar 2.25 3 4.5 g
  • Mix tryptone and NaCl in water first.

  • Bring to final volume.

  • Divide into volumes suitable for the vessel(s) going into the autoclave. (E.g., 750 mL per 1 Liter bottle.)

  • Add correct amount of agar (3 g per L) to each container. Leave stir bar in container!

  • Autoclave 60 min

  • Sit on stir plate, stirring, until handleable

  • Pour 25 mL per plate. Plates do not keep well, so pour them the morning of the day they will be inoculated.

4 plates per group. (= 48 minimum) So make 1500 mL. Really need 55 plates + some tiny petri dishes to demonstrate swarm agar consistency.

Inoculate at 7 pm: 13 WT plates + 7 of each of the other strains.