Chaos saline

Chaos inorganic medium from stock solutions

salt mM
CaCl2.2H2O 0.5
MgSO4.6H2O 0.05
K2HPO4 0.16
KH2PO4 0.11
To make mL Chaos Saline
add: µL 1M CaCl2.2H2O
add: µL 1M MgSO4.6H2O
add: µL 1M K2HPO4
add: µL 1M KH2PO4

Autoclave

Keep amoebae at 22 - 24C, in the dark.

Each pair of students needs ~60 mL sterile saline

  • 10 mL initial Paramecium culture in culture flask
  • 50 mL to set up further cultures for experiments