Start overnight of RNAi plasmid containing bacteria:
- pL4440 empty
- pL4440 + lsy-2
- pL4440 + choice
Plates of bacteria with:
- pL4440 empty
- pL4440 + lsy-2 (chemo assay)
Groups will choose one of the following for phenotypic analysis:
- pL4440 + bli-1
- pL4440 + dpy-5
- pL4440 + unc-22 (from Chase Lab)
10 mL per overnight of sterile LB + 12.5 µg/mL tetracycline
Adding 10 µL of 50 mg/mL ampilicillin stock
- 15 mL conical tubes for overnight, 3 per group, so 36 tubes = 360 mL of broth minimum
- Sterile toothpicks
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