2/6 (M)

2/6 (M) kdorfman Wed, 01/18/2012 - 20:29

Start overnight of RNAi plasmid containing bacteria:

  • pL4440 empty
  • pL4440 + lsy-2
  • pL4440 + choice

Plates of bacteria with:

  • pL4440 empty
  • pL4440 + lsy-2 (chemo assay)

Groups will choose one of the following for phenotypic analysis:

  • pL4440 + bli-1
  • pL4440 + dpy-5
  • pL4440 + unc-22 (from Chase Lab)

10 mL per overnight of sterile LB + 12.5 µg/mL tetracycline

Adding 10 µL of 50 mg/mL ampilicillin stock

  • 15 mL conical tubes for overnight, 3 per group, so 36 tubes = 360 mL of broth minimum
  • Sterile toothpicks