Week 02 - Yeast mating
Week 02 - Yeast mating kdorfman Thu, 07/20/2023 - 18:16Yeast labs, week of 9/11/23 (2nd week of classes, 1st complete week)
See notes here
Total YED plates for the week for 4 sections plus prep: 75
Students mate 3 A mating types with 3 alpha mating types
Take YED plates out early so they stop sweating.
Make an assembly line of 7 stations (use templates):
station | activity | materials |
---|---|---|
1 | label plate | YED plates, labeling template, Fisher Finest marker |
2 | plate HA1 across | 3 small plates of HA1 on YEPAD, sterile toothpicks, used toothpick bucket |
3 | plate HA2 across | 3 small plates of HA2 on YEPAD, sterile toothpicks, used toothpick bucket |
4 | plate HAR across | 3 small plates of HAR on YEPAD, sterile toothpicks, used toothpick bucket |
5 | Plate HB1 down, mixing carefully with the A strain already there, new toothpick each time | 3 small plates of HB1 on YEPAD, sterile toothpicks, used toothpick bucket |
6 | Plate HB2 down | 3 small plates of HB2 on YEPAD, sterile toothpicks, used toothpick bucket |
7 | Plate HBR down | 3 small plates of HBR on YEPAD, sterile toothpicks, used toothpick bucket |
For mating, students need these strains on YEPAD plates so they all start out white:
- HA1
- HB1
- HA2
- HB2
- HAR
- HBR
For observation, students need these strains on YED plates so their colors can be observed:
- HA0
- HB0
- HA1
- HB1
- HA2
- HB2
- HAR
- HBR
For observation of colors, make demo plates (maybe 4 of each):
- 4 YED plate with 4 A strains
- 4 YED plate with 4 alpha (B) strains
(If you let the parental strains sit on YED more than a couple of days, the mutant strains turn red, and the red color will still be there even after there are heterozygous diploids with complementation.)
A types | alpha (B) types |
---|---|
HA0 (WT) | HB0 (WT) |
HA1 (ade1) | HB1 (ade1) |
HA2 (ade2) | HB2 (ade2) |
Start 6 strains at least the Friday before the first lab.
Instructors should make some mating plates to have in the fridge to hand out when student plates are bad.