ATP
ATP kdorfman Wed, 10/26/2011 - 19:2310 mM ATP Stock
(from Maniotis)
60 mg ATP in 8 mL H2O
pH to 7.0 with 0.1 M NaOH
Bring volume to 10 mL with H2O
Aliquot and freeze.
10 µM (=5.731 mg/L) ATP in HBS
Use ATP aliquots from Lab 7.1
10 µM ATP, Calcium-free
Lab 7.2: 0.75 mL per coverslip x 2 coverslips x 7 groups = 10.5 mL
Make 13 mL
13 µL 10 mM ATP stock in 13 mL ca- HBS (1 µL 10 mM ATP stock per 1 mL HBS.)
8 1.6 mL aliquots for Lab 7.2