Bradykinin

1 mg/mL stock
(= 1.0602 mM) Sigma B3259 - 1 mg septum bottle
MW = 1060.2
Add 1 mL H2O to the 1 mg in the bottle (NO CALCIUM! - some will be used for the calcium experiments in Lab 7.2)

Aliquots are 21.2 µL.

2 µM solution for use
(=2.12 µg/mL)

NOTE: Rather weak response in 2011. Try doubling the concentration?

Make enough for Labs 7.1 and 7.2:

• Lab 7.1: 0.750 mL per coverslip x 4 coverslips x 7 groups = 21 mL
• Lab 7.2: 0.75 mL per coverslip x 2 coverslips x 7 groups = 10.5 mL

Make 40 mL

~2 µL 1 mg/mL stock per 1 mL working solution

Each 21.2 µL aliquot of 1 mg/mL makes 10 mL 2µM working solution

84.8 µL 1 mg/mL stock in 40 mL HBS

Make 24 aliquots of 1.6 mL (16 for Lab 7.1, 8 for Lab 7.2)

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4 µM solution for use
(=4.24 µg/mL)

NOTE: Double the 2011 concentration

Make enough for Labs 7.1 and 7.2:

• Lab 7.1: 0.750 mL per coverslip x 4 coverslips x 7 groups = 21 mL
• Lab 7.2: 0.75 mL per coverslip x 2 coverslips x 7 groups = 10.5 mL

Make 40 mL

~4 µL 1 mg/mL stock per 1 mL working solution

Each 21.2 µL aliquot of 1 mg/mL makes 5 mL 2µM working solution

169.6 µL 1 mg/mL stock in 40 mL HBS (8 aliquots)

Make 24 aliquots of 1.6 mL (16 for Lab 7.1, 8 for Lab 7.2)