Lab 5.1 prep
Lab 5.1 prep kdorfman Wed, 08/25/2021 - 20:4811/18 & 11/30 2021
11/18 Thursday
Group 1: Botros et al.
- Zone of inhibition
- E. coli Addgene 20972 from molecular biology experiment
- LB Amp 25 mL
- alcohol (ethanol or isopropanol?)
- disinfectant??
- 20 LB amp plates for zone of inhibition
- sterile discs
- sterile forceps
Group 2 (Vengalil et al.):
- E. coli Addgene 20972 from molecular biology experiment grown in LB-amp
- LB amp salt-free
- LB amp 2M NaCl
- add 2.922 g NaCl per 25 mL salt-free LB
- LB amp 2M KCl
- add 14.9 g KCl per 100 mL LB
- 20 LB amp plates
Group 3 (Long, et al.):
- 40 LB plates (no antibiotic)
- Replica plate materials
- Kid's hand sanitizer
- room swipe access (need names and Spire IDs)
Group 4 ( Lee, et al.):
- E. coli GFP culture in LB Kan
- LB Kan ~25 mL
- LB-glu Kan ~15 mL
- 96 well black plate, shared with group 5
- rewrite script for only the rows they use
Group 5 (Creto, et al.)
- E coli GFP culture in LB Kan
- LB Kan ~25 mL
- LB-glu Kan ~15 mL
- LB-lac Kan ~15 mL
- LB-gal Kan ~15 mL
- 96 well black plate, shared with group 4
- rewrite script for only the rows they use
11/30 TUESDAY
McKnight, Carson, Matfes, Berke
- change pH of LB
- GFP expression
- plate reader
Bowen, Howall, Abraham, Wald
- fertilizer - N-based vs P-based.
- Nitrogen vs phosphorus fertilizer
- LB,
- sterile tubes,
- plates
- Culture (non-GFP?)
Boyd, Ebian, Melusen, Manning
- pH again
Cloutier, Dawiczyk, Tan, Joyce
- mouthwash
- plate reader
- culture (non-GFP?)
- Liquid LB
Alexander, Ferriera, Beinstein, Birnbach
- comparison of various substances at killing E. coli
- LB broth and plates
- E coli culture (not GFP?)
Crotty, Galligan, Horrigan, Jansen
- NaCl
- KCl
- plate reader overnight
- agar plates