minimal medium (glycerol)

minimal medium (glycerol) bcrcstaff Wed, 11/14/2018 - 17:34

For motility strains

To make mL min med
start with mL 10X M9
add: mL 200X HMLTT
add: mL 100% glycerol
add: mL H2O
add: mL 1M MgSO4
add: uL 1M CaCl2, stir if it precipitates
Bring to final volume