LPGM LPGM kdorfman Wed, 08/31/2016 - 20:24 Lilly pollen growth medium 205 mM (7%) sucrose (See Sugars.) 1.6 mM H3BO3 0.1 mM CaCl2 15 mM MES To make mL LPGMat % sucroseadd: mL 150 mM MESadd: uL 10 mM CaCl2add: uL 160 mM Borate, pH to 5.7 with KOHadd: g sucrose Either make fresh each day, or filter sterilize for all the labs. Stock solutions: 150 mM MES 10 mM CaCl2 160 mM Borate