Ionomycin for 6.2
Ionomycin for 6.2 kdorfman Mon, 11/04/2013 - 17:24Ionomycin stock
Fisher or Invitrogen (Life Technologies) I24222
1 mM in DMSO or EtOH. 1 mg makes 1.34 mL stock.
3 µM Ionomycin in HBS
Dilute stock 1:333 in HBS: 3 µL/1 mL
24 µL in 8 mL, aliquot 800 µL
They only need 750µL, because they do one experiment in HBS, then two in Ca-free HBS.
3 µM Ionomycin in Ca-free HBS
Dilute stock 1:333 in Ca-free HBS: 3 µL/1 mL
45 µL in 15 mL
Takes a long time to thaw. Vortex.
Aliquot 1.55 mL so they can do two 750 µL experiments
Vasopressin for 6.2
Vasopressin for 6.2 kdorfman Mon, 11/04/2013 - 17:261 mg/mL (= 0.9225 mM) Vasopressin stock in water
1 µM dilution to make student solutions
1 µL 1 mg/mL in 922.5 µL water (NO CALCIUM, so stock can be used for Ca-free solutions )
10 nM (= 1.084 µg/mL) Vasopressin in HBS for students
Use Vasopressin aliquots from Lab 6.1
Calcium Free Vasopressin 10 nM
10 nM (= 1.084 µg/mL) for students
1 part 1µM dilution to 99 parts Ca-free HBS
Lab 7.2: 0.75 mL per coverslip x 2 coverslips x 8 groups = 12 mL
Make 15 mL: 150 µL + 15 mL Ca-free HBS
Make 8 aliquots of 1.6 mL