Paraformaldehyde Fix

Paraformaldehyde Fix kdorfman Sun, 10/27/2013 - 19:32

3.7% paraformaldehyde, 0.5% Triton-X
in PBS

Paraformaldehyde stock: 32%
Triton X stock: 10%

EMS RT 15714

To make mL fixative
add: mL 32% paraformaldehyde
add: mL 10% triton-X
add: mL 10X PBS

To fix the adherent cells (still in hood):