Antibiotics 2013
Antibiotics 2013 kdorfman Mon, 01/28/2013 - 19:48(weight indicates how much should be on one disk.)
Calculate concentration from volume needed per disk (25 uL)
Make 35 mL of each (because the filter syringe only holds that much)
See recipes in stock solutions.
Make 35 mL each, make 48 0.7 mL aliquots
| Antibiotic | ug/disk | mg/mL | category | solubility | Fisher # |
|---|---|---|---|---|---|
| Ampicillin | 10 | 0.4 | beta-lactam | 50 mg/mL in 1 M NH4OH | |
| tetracycline | 30 | 1.2 | tetracycline | 10 mg/mL in water | |
| cipro | 5 | 0.2 | quinolone | 30 mg/mL in water | |
| Kanamycin | 30 | 1.2 | Aminoglycoside | 50 mg/mL in water |
cipro solubility http://www.inspiralis.com/go/antibiotics.php 30 mg/mL in water
ampicillin solubility 50MG/ML IN 1M AMMONIUM HYDROXIDE http://www.sigmaaldrich.com/catalog/DataSheetPage.do?brandKey=SIGMA&sym… ampicillin sodium salt is soluble 50 mg/mL in water http://www.bioxys.com/i_gentaur/ampicillin_sodium.htm
Kanamycin 50 mg/mL in water
Tetracycline 10 mg/mL
The HCl forms are more water soluble