Extraction prep
Extraction prep kdorfman Mon, 04/02/2012 - 19:05Equipment
- razor blades
- transilluminators
- face shields
- cutting boards
- dry bath at 50C
- columns & collection tubes
Solutions
- Add ethanol to Buffer PE
- Buffer QG ~2 mL (steps 4 & 8)
- isopropyl - ~0.5 mL
- NaOAc 3M, pH 5.0 ~25 µL
- Buffer PE ~ 1.75 mL
- Buffer EB ~ 75 µL (=Tris-Cl 10 mM pH 8.5)
- 6x loading dye
- 6 gels 1.5% (= 300 mL TAE + 4.5 g agarose + 3 µL EtBr)