Soution A
Soution A kdorfman Mon, 03/12/2012 - 18:00Solution A
100 mM Tris-HCl pH 7.5
100mM EDTA
100 mM NaCl
0.5% SDS
| Stock solution | 10 | 25 | 50 | 100 | mL final volume |
|---|---|---|---|---|---|
| 1 M Tris | 1 | 2.5 | 5 | 10 | mL |
| 0.5 M EDTA | 2 | 5 | 10 | 20 | mL |
| 5 M NaCl | 0.02 | 0.05 | 0.1 | 0.2 | mL |
| 20% SDS | 0.25 | 0.625 | 1.25 | 2.5 | mL |
Make 10 mL
pH to 7.5