PIPES
PIPES kdorfman Thu, 01/19/2012 - 16:430.5 M pH 6.7
(for transformation buffer for genetics)
MW (disodium salt) = 346.32
173.16 g/L
17.316 g/ 100 mL
Mix in ~80% of final volume
pH to 6.7 with 5M KOH
Filter sterilize
Aliquot
Freeze
0.5 M pH 6.7
(for transformation buffer for genetics)
MW (disodium salt) = 346.32
173.16 g/L
17.316 g/ 100 mL
Mix in ~80% of final volume
pH to 6.7 with 5M KOH
Filter sterilize
Aliquot
Freeze