QSB antibiotics
QSB antibiotics kdorfman Thu, 01/12/2012 - 14:40From Peg's list (weight indicates how much should be on one disk.)
Calculate concentration from volume needed per disk (25 uL)
Make 35 mL of each (because the filter syringe only holds that much)
See recipes in stock solutions.
Make 35 mL each, make 48 0.7 mL aliquots
| Antibiotic | ug/disk | mg/mL | category | solubility | Fisher # |
|---|---|---|---|---|---|
| Ampicillin | 10 | 0.4 | beta-lactam | 50 mg/mL in 1 M NH4OH | |
| Erythromycin | 15 | 0.6 | macrolide | 50 mg/mL in EtOH | ICN19452901 1g $33.68 |
| tetracycline | 30 | 1.2 | tetracycline | 10 mg/mL in water | |
| cipro | 5 | 0.2 | quinolone | 30 mg/mL in water | |
| Kanamycin | 30 | 1.2 | Aminoglycoside | 50 mg/mL in water |
cipro solubility http://www.inspiralis.com/go/antibiotics.php 30 mg/mL in water
ampicillin solubility 50MG/ML IN 1M AMMONIUM HYDROXIDE http://www.sigmaaldrich.com/catalog/DataSheetPage.do?brandKey=SIGMA&sym… ampicillin sodium salt is soluble 50 mg/mL in water http://www.bioxys.com/i_gentaur/ampicillin_sodium.htm
erythromycin solubility 50MG/ML IN ETHANOL http://www.sigmaaldrich.com/catalog/DataSheetPage.do?brandKey=SIGMA&sym…
Kanamycin 50 mg/mL in water
Tetracycline 10 mg/mL
The HCl forms are more water soluble
Not using Spring 2012
| Antibiotic | ug/disk | mg/mL | category | Fisher # |
|---|---|---|---|---|
| Amikacin | 30 | BP2643250 250 mg $28 | ||
| Cefazolin | 30 | NC9863037 1g/10mL $17 | ||
| gentamicin | 10 | ICN1676045 10mg/mL 10 mL $29 | ||
| ticarcillin | 7 | 50-121-7983 1 g $45 | ||
| trimethoprim | 5 | ICN19552701 1 g $34 | ||
| tobramycin | 10 | 50-146-785 100 mg $56 |