Microscopes
Microscopes kdorfman Mon, 01/09/2012 - 15:20Nikon Filter Cubes
| label on filter wheel | excitation wavelength | emission wavelength | used for | label on cube |
|---|---|---|---|---|
| UV | UV (360 nm) | blue (460 nm) | DAPI | UV-2E-C |
| B | B (480 nm) | green (535 nm) | GFP, fluorescein | B-2E/C |
| G | G ( 560 nm) | red (630 nm) | Texas red, rhodamine | Y-2E/C |
| LP | B (470 nm) | long pass green - red (>500 nm) | chlorophyll & GFP simultaneously | B-2A |
| mOrange1 | G (530 nm) | orange (575). | m orange | Chroma 49014 |
Details:
Ultraviolet Excitation Filter Block UV-2E/C Specifications:
Excitation Filter Wavelengths: 340-380 nanometers (bandpass, 360 CWL)
Dichromatic Mirror Cut-on Wavelength: 400 nanometers (longpass, LP)
Barrier Filter Wavelengths: 435-485 nanometers (bandpass, 460 CWL)
Blue Excitation Filter Block B-2E/C Specifications:
Excitation Filter Wavelengths: 465-495 nanometers (bandpass, 480 CWL)
Dichromatic Mirror Cut-on Wavelength: 505 nanometers (longpass, LP)
Barrier Filter Wavelengths: 515-555 nanometers (bandpass, 535 CWL)
Yellow Excitation Filter Block Y-2E/C Specifications:
Excitation Filter Wavelengths: 540-580 nanometers (bandpass, 560 CWL)
Dichromatic Mirror Cut-on Wavelength: 595 nanometers (longpass, LP)
Barrier Filter Wavelengths: 600-660 nanometers (bandpass, 630 CWL)
Blue Excitation Filter Block B-2A Specifications:
Excitation Filter Wavelengths: 450-490 nanometers (bandpass, 470 CWL)
Dichromatic Mirror Cut-on Wavelength: 500 nanometers (longpass, LP)
Barrier Filter Wavelengths: 515 nanometer cut-on (longpass, LP)
mKO/mOrange Specifications:
Excitation Filter Wavelengths: 515-545 nm
Dichromatic Mirror cut-on wavelength: 550 nm
Barrier Filter Wavelengths: 555 - 595 nm
NIkon repairs (every summer)
contact John DeToma: johnd@mvi-inc.com
They need a PO before they schedule an on-site service appointment
-
installed on scope 7 for Akiko Okusu's class ↩︎
Aligning the xenon arc lamp
Aligning the xenon arc lamp kdorfman Wed, 04/10/2019 - 18:34DIC
DIC bcrcstaff Tue, 11/20/2018 - 15:29(On microscope 7)
Prism combinations
| objective | magnification | prism on condenser turret | prism on objective turret |
|---|---|---|---|
| Plan Fluor phase | 10x | N1 | 10x |
| Plan Fluor phase | 40x (short working distance) | N2 | 40x I |
| S Plan Fluor phase | 40x (extra-long WD) | N2 | 40x III |
| Plan Fluor phase | 100x oil | N2 | 100x II |
| Achromat w/iris brightfield | 100x oil | no DIC |
