Antibiotics
Antibiotics kdorfman Wed, 12/28/2011 - 21:01Amphotericin
Amphotericin kdorfman Tue, 01/09/2018 - 18:29Anti-Fungal
Stock solution: 10 mg/mL in DMSO (insoluble in ethanol)
Store at 4C short term, -20 long term (in 15 mL tube, wrapped in foil with tape label)
Keep in the dark.
Final concentration in medium = 10 ug/mL
Add 1 mL per L
Freezes in the refrigerator.
Protect from light.
Ampicillin
Ampicillin kdorfman Wed, 12/28/2011 - 21:0250 mg/mL stock
(Final concentration in medium = 50 µg/mL)
Lasts up to 4 weeks in poured agar at 4C
0.5 g in 10 mL water
filter sterilize
1 mL aliquots (enough for 1 L medium)
0.4 mg/mL for QSB
Make 35 mL: 0.28 mL 50 mg/mL stock
35 mL sterile water
(filter sterilize if any doubt about sterility)
48 0.7 mL aliquots
Can also use carbenicillin
Carbenicillin
Carbenicillin kdorfman Tue, 08/02/2016 - 15:05(More stable than ampicillin)
Fisher 50841234b 25 mL Teknova C2130
100 mg/mL stock
effective concentration 50 - 100 µg/mL in medium
Freeze aliquots.
Chloramphenicol
Chloramphenicol kdorfman Thu, 04/23/2015 - 13:37Cipro
Cipro kdorfman Wed, 12/28/2011 - 21:02Ciprofloxacin 10 mg/mL stock
(final concentration in medium = 10 µg/mL)
100 mg in 10 mL dilute acid (add HCl drop by drop, mixing in between till it dissolves)
filter sterilize
1 mL aliquots
freeze
0.2 mg/mL for QSB
0.7 mL 10 mg/mL stock
35 mL water
filter sterilize
48 0.7 mL aliquots
freeze
Doxycycline
Doxycycline kdorfman Mon, 01/18/2016 - 13:27Tetracycline category
solubility 50 mg/mL in water
disk has 30 ug
Erythromycin
Erythromycin kdorfman Fri, 01/20/2012 - 18:10solubility
50 mg/mL in ethanol
lasts up to 4 weeks in poured agar at 4C
10 mg/mL stock
(10 µg/mL in medium)
0.25 g in 25 mL EtOH
1 mL aliquots
freeze
0.6 mg/mL for QSB
2.1 mL 10 mg/mL stock
33 mL EtOH1
48 0.7 mL aliquots
freeze
-
Try 20 µL in 330 µL water to see if it dissolves. If so, then make aqueous solution, filter sterilize, and freeze. ↩︎
Kanamycin
Kanamycin kdorfman Wed, 12/28/2011 - 21:0150 mg/mL stock (50 µg/mL in medium)
Concentration in poured agar drops to ~80% in 1 week, but stays stable for 3 more weeks at 4C
1.25 g in 25 mL H2O
filter sterilize
mL aliquots
freeze
1.2 mg/mL for QSB
0.84 mL 50 mg/mL stock
34.16 mL water
filter sterilize (if any doubt about sterility)
48 0.7 mL aliquots
freeze
Kan calcs
Kan calcs kdorfman Tue, 10/02/2012 - 16:23Streptomycin
Streptomycin kdorfman Tue, 10/17/2023 - 19:43In refrigerator in 362A
Fisher Streptomycin sulfate 50g
BP910-50 (discontinued)
MW 1457.58
~50 mg/L for agar
Tetracycline
Tetracycline kdorfman Wed, 01/18/2012 - 20:28Standard is 10 mg/mL stock
Stable for 2 weeks in poured agar at 4C; 75% after 4 weeks
15 mg/mL stock
0.275 g tetracycline in 25 mL EtOH
1 mL aliquots
freeze
1.2 mg/mL for QSB
2.8 mL 15 mg/mL stock in 32.2 mL EtOH1
48 0.7 mL aliquots
freeze
-
Try 28 µL in 322 µL water to see if it dissolves. If so, then make aqueous solution, filter sterilize, and freeze. ↩︎
Triclosan
Triclosan kdorfman Wed, 12/28/2011 - 21:02Irgasan
Sigma 72779-5g-f
MW 289.54