10/17/23

10/17/23 kdorfman Mon, 10/16/2023 - 19:26

Trial run for Lamin antibodies

Fix 15 min in paraglut

1o Ab: Rabbit anti-lamin B

  • dilute 1:1000 with PBS-Tw-Az-BSA
  • 2 ┬ÁL antibody into 2 mL buffer
  • 1 hr 37C

2o Ab: goat anti-rabbit

  • 1 hr RT incubation
Cell type # coverslips
MEF WT NLS-GFP (40) 3
HCT-116 WT (60) 3
DU-145 (74) 4

Stain with Hoechst (1:10,000) in the final PBS-Tw-Az rinse

Mount with non-DAPI Fluoromount (Fisher OB100-01)